Identification ofTrichodermastrains from building materials by ITS1 ribotyping, UP-PCR fingerprinting and UP-PCR cross hybridization
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چکیده
منابع مشابه
Application of PCR ribotyping and tDNA-PCR for Klebsiella pneumoniae identification.
PCR analysis of 16S-23S internal transcribed spacer (PCR ribotyping) and tRNA intergenic spacer (tDNA-PCR) were evaluated for their effectiveness in identification of clinical strains of Klebsiella pneumoniae and differentiation with related species. For this purpose both methods were applied to forty-three clinical isolates biochemically identified as K. pneumoniae subsp. pneumoniae isolated f...
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INTRODUCTIONDevelopment of the polymerase chain reaction (PCR) as a basic component of the molecular biology laboratory has occurred very rapidly from its inception in 1985. As PCR became more widely used, scientists rapidly learned more about it and, as a result, learned that PCR had its strong points and its deficiencies. Very quickly, PCR demonstrated its power to amplify very small amounts ...
متن کاملPCR-based ribotyping of Staphylococcus aureus.
Genotyping techniques are valuable tools for the epidemiologic study of Staphylococcus aureus infections in the hospital setting. Pulsed-field gel electrophoresis (PFGE) is the current method of choice for S. aureus strain typing. However, the method is laborious and requires expensive equipment. In the present study, we evaluated the natural polymorphism of the genomic 16S-23S rRNA region for ...
متن کاملMolecular Analysis of Toxigenic Clostridium difficile Isolates from Hospital Environment by PCR Ribotyping Method
Background and Aims: Clostridium difficile is an identified cause of antibiotic-associated diarrhea, antibiotic-associated colitis, pseudomembranous colitis and nosocomial diarrhea. The objective of this survey was to determine molecular analysis of toxigenic Clostridium difficile isolates from hospital environment in Tehran tertiary medical centers. Materials and Methods: In this descriptiv...
متن کاملPosttreatment follow-Up of brucellosis by PCR assay.
In order to evaluate the usefulness of a peripheral blood PCR assay in the posttreatment follow-up of brucellosis, a cohort of 30 patients was studied by means of blood cultures, rose Bengal, seroagglutination, Coombs' antibrucella tests, and PCR assay at the time of diagnosis, at the end of treatment, and 2, 4, and 6 months later. Of the 29 patients whose PCR assays were initially positive, 28...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2000
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2000.tb09050.x